Synovial Sarcoma Microvesicles Harbor the SYT-SSX Fusion Gene Transcript: Comparison of Different Methods of Detection and Implications in Biomarker Research

Figure 7

Analysis of the presence of the SYT-SSX fusion gene in whole blood, the mononuclear cell fraction, and serum microvesicles of synovial sarcoma patients by nested PCR. THP-1 cells: negative control. 1273/99 synovial sarcoma cells: positive control, showing the SYT-SSX fusion gene transcript (212 bp; the primers were designed to amplify both SYT-SSX1 and SYT-SSX2 subtypes [20]).