Stem Cells International

Research Article

Synovial Sarcoma Microvesicles Harbor the SYT-SSX Fusion Gene Transcript: Comparison of Different Methods of Detection and Implications in Biomarker Research

Table 2

Comparison of sensitivity of nested PCR, qPCR, nested PCR, and ddPCR at detection of SYT-SSX fusion gene in 1273/99 synovial sarcoma microvesicles. D: detected, ND: not detected.
Amount of total RNA in ng (synovial sarcoma microvesicles)Nested qPCRqPCRNested PCRddPCR
25,000DDDD
12,500DDDD
6,250DDDD
3,125DDDND
1,562DDNDND
0,781DDNDND
0,391DNDNDND
0,195DNDNDND
0,098DNDNDND
0,049DNDNDND
0,024DNDNDND
0,012DNDNDND
0,006DNDNDND
0,003NDNDNDND