Characterization of AD-MSCs and the expression pattern of TLRs. (a) The cell morphology of AD-MSCs observed under light microscope. (b) Immunophenotype of AD-MSCs. (c) Differentiation capacity of AD-MSCs was demonstrated by ALP staining (left) and Alizarin red staining (centered) for osteoblasts and oil red O staining for adipocytes (right). (d) The expression levels of TLRs were analyzed by real-time PCR.