Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR

Figure 1

(a), (b), (c), and (d) Alizarin Red S Staining of GSC and ASC after 21 days of osteoblast induction. (a) and (b) GSC and ASC were cultured in proliferation medium. No differentiation is noticed. (c) and (d) GSC and ASC were cultured in osteogenic medium. Calcium mineral deposits confirmed osteoblast differentiation in both stem cells after 21 days of culture. Bar scale = 100 μm. (e) Colony forming units for GSCs. Limiting dilution of GSCs shows their ability to form colonies after 5 days of culture. (f) Agarose gel electrophoresis: total RNA quality. All RNA samples showed absence of degradation and a high degree of integrity. Upper bands: 28S, lower bands: 18S, and lanes 1 to 4: random RNA samples from GSC and ASC.

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