Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR

Figure 2

PCR reaction values and expression levels of the 10 candidate reference genes. (a) Melt peak temperature was determined for each reference gene to confirm the specificity of each primer. (b) Threshold values were manually set for each reference gene to calculate cycle threshold (Ct) values. (c), (d), and (e) Expression levels (Ct values) were determined for all reference genes throughout all the samples: (c) GSCs (), (d) dGSCs (), and (e) ASCs (). The central bars correspond to the mean Ct values; the upper and lower bars represent the standard deviation. These values were used in Ct and BestKeeper algorithms.

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