Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR

Figure 4

Gene expression stability and minimal number of genes needed in (a) GSC (), (b) dGSC (), and (c) ASC (), by GeNorm. ((a1), (a2), and (a3)) Average expression stability values () for GSC, dGSC, and ASC. A lower value indicated a more stable expression. ((b1), (b2), and (b3)) Pairwise variation value below 0.16 with the least number of reference candidates used is considered optimal for GSC, dGSC, and ASC.

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