Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR

Figure 8

Effect of the choice of stable HKGs in the study of dGSC () (a) and ASC () (b). The normalization of fold expression of an osteogenic marker, RUNX2, was performed on D0, D7, and D14. As calibrators: for dGSCs: TBP/SDHA the most stable HKGs and RPS18 the least stable one. For ASCs: SDHA/HPRT1 the most stable HKGs and ACTB the least stable one. values have been obtained with unpaired -test. , , and and “ns” stands for .

(a)

(b)