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Stem Cells International
Volume 2016, Article ID 6261490, 17 pages
Research Article

Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR

1Laboratory of Molecular Oral Physiopathology, INSERM UMRS 1138, Cordeliers Research Center, 75006 Paris, France
2Paris-Descartes, Pierre and Marie Curie, and Paris-Diderot Universities, UFR Odontology, 75006 Paris, France
3AP-HP, Hospital Complex Henri-Mondor Albert-Chenevier, CIC-BT-504, 94000 Creteil, France
4Reference Center for Dental Rare Disease, Rothschild Hospital, 75012 Paris, France

Received 2 July 2015; Accepted 4 October 2015

Academic Editor: Yupo Ma

Copyright © 2016 Ihsène Taïhi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material 1: Three tables showing normalized values (R) of the three groups of samples GSCs, dGSCs and ASCs with the ten studied HKGs. R were calculated using formula and depend on E the efficiency of each primer (1).

Supplementary Material 2: Two tables describing the ΔCt comprative method for dGSCs and ASCs and the ranking of the ten HKGs according to StdDev mean of each gene.

Supplementary Material 3: Mineral nodules were observed for dGSCs at different time points D7, D14 and D21. This confirmed the osteogenic differentiation in accordance with the increase of RUNX2 gene expression. Alizarin Red S staining at D21 confirmed the calcium content of these nodules.

Supplementary Material 4: MIQE checklist was almost respected by the different steps of our study, from collecting samples to gene expression analyses, which is in accordance with the accuracy of our results.

  1. Supplementary Materials