Research Article

Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR

Table 1

10 candidate housekeeping genes analysed.

Gene symbolGene nameGene accession numberPrimer sequence (F/R) (%)Amplicon
size (bp)

TBPTATA box binding proteinNM_003194.4 F: CAC GAA CCA CGG CAC TGA TT94,288
R: TTT TCT TGC TGC CAG TCT GGA C

SDHASuccinate dehydrogenase complex, subunit A, flavoproteinNM_004168.3F: AGC AAG CTC TAT GGA GAC CT88,4199
R: TAA TCG TAC TCA TCA ATC CG

HPRT1Hypoxanthine phosphoribosyltransferase INM_000194.2F: TGT TGG ATT TGA AAT TCC AGA CAA G90,5107
R: CTT TTC CAG TTT CAC TAA TGA CAC AA

GAPDHGlyceraldehyde-3-phosphate dehydrogenaseNM_002046.5F: CTT TGT CAA GCT CAT TTC CTG GTA82,570
R: GGC CAT GAG GTC CAC CA

RPS18Ribosomal protein S18NM_022551.2F: AGC TTG TTG TCC AGA CCA TT86,7188
R: TGA GGA AAG CAG ACA TTG AC

ALAS15′-Aminolevulinate synthase 1NM_000688.5F: AAC TTC CCA AAA TCT GTT TC88,8158
R: GGT GAT GAG GGA GTC TGA AT

ACTBActin, beta NM_001101.3F: CTG TGG CAT CCA CGA AAC9688
R: CAG ACA GCA CTG TGT TGG

B2MBeta-2-microglobulinNM_004048.2 F: CAG CAT CAT GGA GGT TTG AA92178
R: TGG AGA CAG CAC TCA AAG TA

UBCUbiquitin CNM_021009.6F: GTG GCA CAG CTA GTT CCG T10396
R: CTT CAC GAA GAT CTG CAT TGT CA

RPII50S ribosomal protein L9NM_031420.3F: CTT CAC GGT GCT GGG CAT T95240
R: GTG CGG CTG CTT CCA TAA