Stem Cells International

Research Article

Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR

Table 3

The ΔCt comparative method analysis for GSCs.
Gene versus geneMean ΔCtStdDevMean StdDevGene versus GeneMean ΔCtStdDevMean StdDev
TBP versus SDHA3,730,84 1.05ALAS1 versus TBP0,561,51 1.27
TBP versus HPRT11,850,86ALAS1 versus SDHA−3,181,02
TBP versus GAPDH8,480,92ALAS1 versus HPRT1−1,301,87
TBP versus RPS188,991,00ALAS1 versus GAPDH−7,931,27
TBP versus ALAS10,561,51ALAS1 versus RPS18−8,431,37
TBP versus ACTB8,670,85ALAS1 versus ACTB8,121,20
TBP versus B2M9,290,88ALAS1 versus B2M8,730,99
TBP versus UBC−1,680,64ALAS1 versus UBC−2,241,36
TBP versus RPII0,991,99ALAS1 versus RPII0,430,85
SDHA versus TBP3,730,84 0.97ACTB versus TBP8,670,85 0.98
SDHA versus HPRT1−1,881,36ACTB versus SDHA4,940,63
SDHA versus GAPDH4,750,75ACTB versus HPRT16,821,01
SDHA versus RPS185,251,00ACTB versus GAPDH0,190,93
SDHA versus ALAS1−3,181,02 ACTB versus RPS18−0,311,18
SDHA versus ACTB4,940,63ACTB versus ALAS18,121,20
SDHA versus B2M5,560,90ACTB versus B2M0,620,83
SDHA versus UBC−5,410,89ACTB versus UBC−10,350,67
SDHA versus RPII−2,751,33ACTB versus RPII−7,691,54
HPRT1 versus TBP1,850,86 1.37B2M versus TBP9,290,88 0.98
HPRT1 versus SDHA−1,881,36B2M versus SDHA5,560,90
HPRT1 versus GAPDH6,631,42B2M versus HPRT17,441,27
HPRT1 versus RPS187,131,23B2M versus GAPDH0,810,72
HPRT1 versus ALAS1−1,301,87B2M versus RPS180,301,09
HPRT1 versus ACTB6,821,01B2M versus ALAS18,730,99
HPRT1 versus B2M7,441,27B2M versus ACTB0,620,83
HPRT1 versus UBC−3,531,03B2M versus UBC−10,970,70
HPRT1 versus RPII−0,872,41B2M versus RPII−8,301,47
GAPDH versus TBP8,480,92 1.06UBC versus TBP−1,680,64 1.02
GAPDH versus SDHA4,750,75UBC versus SDHA−5,410,89
GAPDH versus HPRT16,631,42UBC versus HPRT1−3,531,03
GAPDH versus RSP180,501,16UBC versus GAPDH−10,160,86
GAPDH versus ALAS1−7,931,27UBC versus RPS18−10,661,39
GAPDH versus ACTB0,190,93UBC versus ALAS1−2,241,36
GAPDH versus B2M0,810,72UBC versus ACTB−10,350,67
GAPDH versus UBC−10,160,86UBC versus B2M−10,970,70
GAPDH versus RPII−7,501,54UBC versus RPII2,671,68
RPS18 versus TBP8,991,00 1.27RPII versus TBP0,991,99 1.64
RPS18 versus SDHA5,251,00RPII versus SDHA−2,751,33
RPS18 versus HPRT17,131,23RPII versus HPRT1−0,872,41
RPS18 versus GAPDH0,501,16RPII versus GAPDH−7,501,54
RPS18 versus ALAS1−8,431,37RPII versus RPS18−8,002,00
RPS18 versus ACTIN−0,311,18RPII versus ALAS10,430,85
RPS18 versus B2M0,301,09RPII versus ACTB−7,691,54
RPS18 versus UBC−10,661,39RPII versus B2M−8,301,47
RPS18 versus RPII−8,002,00RPII versus UBC2,671,68