Research Article

Osteogenic Differentiation of Human Amniotic Fluid Mesenchymal Stem Cells Is Determined by Epigenetic Changes

Figure 4

Epigenetic changes during osteogenic differentiation of AF-MSCs. Total proteins were isolated from untreated AF-MSCs (noted as C) and AF-MS cells at day 5 (noted as 5 d) and at day 15 (noted as 15 d) after induction of osteogenic differentiation. (a) Changes in the expression of PRC2 proteins, EZH2, SUZ12, DNMT1, HDAC1, and HDAC2. (b) Changes of histone modifications, H3K4me3, H3K9Ac, H4 hyperAc, and H3K27me3, levels. Numbers below blots show relative density of each band normalized to GAPDH loading control as measured using ImageJ software (NIH, USA). The data represent one of three independent experiments showing similar results.