Research Article
Osteogenic Differentiation of Human Amniotic Fluid Mesenchymal Stem Cells Is Determined by Epigenetic Changes
| Gene | Primer sequence (5′-3′) | Product size (bp) |
| Oct4 | Forward 5′-CGAGAAGGATGTGGTCCGAG | 136 | Reverse 5′-CAGAGGAAAGGACACTGGTC |
| Nanog | Forward 5′-AGATGCCTCACACGGAGACT | 96 | Reverse 5′-GTTTGCCTTTGGGACTGGTG |
| Sox2 | Forward 5′-TGGACAGTTACGCGCACAT | 215 | Reverse 5′-CGAGTAGGACATGCTGTAGGT |
| Rex1 | Forward 5′-GCCTTATGTGATGGCTATGTGT | 96 | Reverse 5′-ACCCCTTATGACGCATTCTATGT |
| Alkaline phosphatase (ALP) | Forward 5′-AGCCCTTCACTGCCATCCTGT | 68 | Reverse 5′-ATTCTCTCGTTCACCGCCCAC |
| Osteopontin | Forward 5′-GAAGTTTCGCAGACCTGACAT | 91 | Reverse 5′-GTATGCACCATTCAACTCCTCG |
| GAPDH | Forward 5′-AGTCCCTGCCACACTCAG | 123 | Reverse 5′-TACTTTATTGATGGTACATGACAAGG |
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