Research Article

Adiponectin Isoforms and Leptin Impact on Rheumatoid Adipose Mesenchymal Stem Cells Function

Figure 3

Effects of unstimulated and HMW/MMW adiponectin-stimulated RA-ASCs on proliferation of PBMCs from healthy donors and IL-10 secretion by these cells. PBMCs either were prestimulated with 2.5 μg/mL of phytohaemagglutinin (activated PBMCs) or were left untreated (resting PBMCs) and then cultured in the presence of unstimulated ASCs (+Ctrl-ASCs) or ASCs stimulated with 10 μg/mL of HMW/MMW adiponectin (+H/M-ASCs). Resting and activated PBMCs cultured separately in DMEM/F12 medium served as controls (DMEM/F12). Proliferation of cells was determined by 3H-thymidine incorporation assay. Radioactivity of samples is expressed in counts per minute (cpm). Concentration of IL-10 was measured in culture supernatants by specific ELISA test. (a) Proliferation of PBMCs cocultured in the presence of ASCs (). (b) IL-10 secretion in ASCs-PBMCs cocultures (). Asterisks () indicate statistically significant differences versus activated (a) or resting (b) PBMCs cultured separately; ; ; N.S.: nonsignificant.