Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2016, Article ID 6549347, 9 pages
http://dx.doi.org/10.1155/2016/6549347
Research Article

Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage

Comprehensive Hearing Center, Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Würzburg, Josef-Schneider-Strasse 11, 97080 Würzburg, Germany

Received 31 January 2016; Revised 5 April 2016; Accepted 27 April 2016

Academic Editor: Kouichi Hasegawa

Copyright © 2016 P. Schendzielorz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006. View at Publisher · View at Google Scholar · View at Scopus
  2. K. Hemmrich, M. Meersch, D. von Heimburg, and N. Pallua, “Applicability of the dyes CFSE, CM-DiI and PKH26 for tracking of human preadipocytes to evaluate adipose tissue engineering,” Cells, Tissues, Organs, vol. 184, no. 3-4, pp. 117–127, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. K. Froelich, G. Steussloff, K. Schmidt et al., “Dil labeling of human adipose-derived stem cells: evaluation of DNA damage, toxicity and functional impairment,” Cells Tissues Organs, vol. 197, no. 5, pp. 384–398, 2013. View at Publisher · View at Google Scholar · View at Scopus
  4. D. Loeffler and S. Ratner, “In vivo localization of lymphocytes labelled with low concentrations of Hoechst 33342,” Journal of Immunological Methods, vol. 119, no. 1, pp. 95–101, 1989. View at Publisher · View at Google Scholar · View at Scopus
  5. C. R. Parish, “Fluorescent dyes for lymphocyte migration and proliferation studies,” Immunology and Cell Biology, vol. 77, no. 6, pp. 499–508, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. P. A. Zuk, M. Zhu, H. Mizuno et al., “Multilineage cells from human adipose tissue: implications for cell-based therapies,” Tissue Engineering, vol. 7, no. 2, pp. 211–228, 2001. View at Publisher · View at Google Scholar · View at Scopus
  7. R. R. Tice, E. Agurell, D. Anderson et al., “Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing,” Environmental and Molecular Mutagenesis, vol. 35, no. 3, pp. 206–221, 2000. View at Google Scholar · View at Scopus
  8. P. L. Olive, R. E. Durand, J. Le Riche, I. A. Olivotto, and S. M. Jackson, “Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers,” Cancer Research, vol. 53, no. 4, pp. 733–736, 1993. View at Google Scholar · View at Scopus
  9. M. F. Pittenger, A. M. Mackay, S. C. Beck et al., “Multilineage potential of adult human mesenchymal stem cells,” Science, vol. 284, no. 5411, pp. 143–147, 1999. View at Publisher · View at Google Scholar · View at Scopus
  10. R. E. Durand and P. L. Olive, “Cytotoxicity, mutagenicity and DNA damage by Hoechst 33342,” The Journal of Histochemistry and Cytochemistry, vol. 30, no. 2, pp. 111–116, 1982. View at Publisher · View at Google Scholar · View at Scopus
  11. N. Mohorko, N. Kregar-Velikonja, G. Repovš, M. Gorensek, and M. Bresjanac, “An in vitro study of Hoechst 33342 redistribution and its effects on cell viability,” Human and Experimental Toxicology, vol. 24, no. 11, pp. 573–580, 2005. View at Publisher · View at Google Scholar · View at Scopus
  12. D. Adamski, J.-F. Mayol, N. Platet, F. Berger, F. Hérodin, and D. Wion, “Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation,” FEBS Letters, vol. 581, no. 16, pp. 3076–3080, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. W. E. Samlowski, B. A. Robertson, B. K. Draper, E. Prystas, and J. R. McGregor, “Effects of supravital fluorochromes used to analyze the in vivo homing of murine lymphocytes on cellular function,” Journal of Immunological Methods, vol. 144, no. 1, pp. 101–115, 1991. View at Publisher · View at Google Scholar · View at Scopus
  14. M. Brenan and C. R. Parish, “Intracellular fluorescent labelling of cells for analysis of lymphocyte migration,” Journal of Immunological Methods, vol. 74, no. 1, pp. 31–38, 1984. View at Publisher · View at Google Scholar · View at Scopus
  15. H. Xue, X.-Y. Zhang, J.-M. Liu, Y. Song, Y.-F. Li, and D. Chen, “Development of a chemically extracted acellular muscle scaffold seeded with amniotic epithelial cells to promote spinal cord repair,” Journal of Biomedical Materials Research Part A, vol. 101, no. 1, pp. 145–156, 2013. View at Publisher · View at Google Scholar · View at Scopus
  16. Y. Chen, J. Qiu, F. Chen, and S. Liu, “Migration of neural precursor cells derived from olfactory bulb in cochlear nucleus exposed to an augmented acoustic environment,” Hearing Research, vol. 228, no. 1-2, pp. 3–10, 2007. View at Publisher · View at Google Scholar · View at Scopus
  17. C. W. Scharenberg, M. A. Harkey, and B. Torok-Storb, “The ABCG2 transporter is an efficient Hoechst 33342 efflux pump and is preferentially expressed by immature human hematopoietic progenitors,” Blood, vol. 99, no. 2, pp. 507–512, 2002. View at Publisher · View at Google Scholar · View at Scopus
  18. D. C. Andersen, H. D. Schrøder, and C. H. Jensen, “Non-cultured adipose-derived CD45- side population cells are enriched for progenitors that give rise to myofibres in vivo,” Experimental Cell Research, vol. 314, no. 16, pp. 2951–2964, 2008. View at Publisher · View at Google Scholar · View at Scopus