Stem Cells International / 2016 / Article / Fig 5

Research Article

Enhanced Proliferation of Porcine Bone Marrow Mesenchymal Stem Cells Induced by Extracellular Calcium is Associated with the Activation of the Calcium-Sensing Receptor and ERK Signaling Pathway

Figure 5

Inhibition of ERK pathway abolished -induced proliferation of pBMSCs. (a) Effects of U0126 (1 μM), an inhibitor of MEK, on the proliferation of pBMSCs after 5-day incubation (). (b) The mRNA expression levels of cyclins (cyclin A2, cyclin D3, and cyclin E2), PCNA, and p21 in response to 4 mM and/or 1 μM U0126. (c) Western blot analysis of cyclin D1, p21, phosphor-ERK (p-ERK), and ERK in pBMSCs after 5-day culture. β-actin was used as loading control. (d) Mean ± SEM of immunoblotting bands of cyclin D1, p21, and p-ERK/ERK. The intensities of the bands were expressed as the arbitrary units (). and versus 1 mM group (control); and versus 4 mM group.

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