FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

Figure 1

Mouse pluripotent stem cell cultures were assayed for relative expression levels of pluripotency (Nanog, SOX2), endodermal (SOX17, Pdx1), and pancreatic (Ins-1, Ins-2, and Gcg) genes at different points in time. RT-qPCR was performed in triplicate for each sample with bars representing means ± SD of 3 biological replicates. Expression levels were normalized against the housekeeping gene ppia. Asterisks () denote statistical significant () increases in pancreatic marker gene expression at specific time points of cultures as compared to nondifferentiated mouse pluripotent stem cells (day 0 of differentiation), # UD represents the undetected genes.