Adipose-Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications

Figure 3

Culturing ASCs with bioactive silicate nanoplatelets promotes the osteogenic differentiation of ASCs. (a) Experimental procedures used to induce osteogenic differentiation. SSEA-4⁺ adipose-derived stem cells (ASCs). SSEA-4⁺ ASCs were seeded in basal medium for 1 day, and silicate nanoplatelets (sNPs) were added to the basal medium. The samples were incubated either in basal medium or under osteogenic differentiating conditions. Samples were collected on days 7, 14, 21, and 28 to analyze differentiation. (b) Qualitative analysis of alkaline phosphatase (ALP) in SSEA-4⁺ ASCs on days 7, 10, 14, and 21 cultured in basal and osteo media, compared to ASCs. The dark purple color increased with sNP concentration level and osteo medium presented a darker purple color. (c) Quantitative analysis of ALP in SSEA-4⁺ ASCs in basal medium with sNPs. The presence of sNPs enhanced ALP activity on day 14, compared to that in ASCs. (d) Quantitative analysis of ALP in SSEA-4⁺ASCs in osteo medium with sNPs. The presence of sNPs enhanced ALP activity on day 14, compared to that in ASCs (adopted from [63]). , , and .

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