TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells

Figure 2

TBX3 knockdown in fibroblasts and keratinocytes during reprogramming causes decreased reprogramming efficiency. (a) Alkaline phosphatase (AP) staining at day 17 of fibroblast reprogramming with a highly significant reduction (23%) of iPSC colonies. (b) Alkaline phosphatase (AP) staining at day 22 of keratinocyte reprogramming with a reduction (91%) of iPSC colonies. (c) Relative expression of OCT4, SOX2, and NANOG during fibroblast reprogramming. (d) Relative expression of OCT4, SOX2, and NANOG during keratinocyte reprogramming.

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