TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells
TBX3 knockdown in fibroblasts and keratinocytes during reprogramming causes decreased reprogramming efficiency. (a) Alkaline phosphatase (AP) staining at day 17 of fibroblast reprogramming with a highly significant reduction (23%) of iPSC colonies. (b) Alkaline phosphatase (AP) staining at day 22 of keratinocyte reprogramming with a reduction (91%) of iPSC colonies. (c) Relative expression of OCT4, SOX2, and NANOG during fibroblast reprogramming. (d) Relative expression of OCT4, SOX2, and NANOG during keratinocyte reprogramming.