Research Article

TBX3 Knockdown Decreases Reprogramming Efficiency of Human Cells

Figure 3

Reprogrammed fibroblasts under TBX3 knockdown have the same stem cell properties as control iPSCs. (a) Pluripotency staining of iPSCs generated from fibroblasts is positive for the pluripotency markers OCT4, SOX2, and NANOG as well as for the surface markers TRA-1-60, TRA-1-81, and SSEA4. (b) Immunocytochemistry of germ layer differentiation shows positive staining for ectoderm (TUBB3), mesoderm (DESMIN), and endoderm (SOX17) for TBX3 knockdown and control, respectively. (c) Expression of ectodermal genes (TUBB3, PAX6), mesodermal genes (T, MYH6), and endodermal genes (AFP, FOXA2) in germ layer differentiation shows pluripotent differentiation potential of the generated iPSCs in both control and TBX3 knockdown cells. Scale bar: 100 μm.