Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method

<div>Histograms of flow cytometry; blue solid filled overlay represents the test sample; red diagonal line filled overlay represents the isotype control. For each histogram the negative gate (red bar) was set for inclusion of 99% of the isotype. Percentages shown in histograms are for the test samples. (a–c) All are markers in the positive cocktail, CD90, CD105, and CD73. The positive gate percentages shown in blue for each sample. (d) The negative cocktail, with CD44 included as a positive control (unfilled overlay) positive percentage in black. (e) CD44 marker included outside of the positive cocktail.</div>

Stem Cells International

fig5

Figure 5

Figure 5: Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method 

Figure 5 | Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method 