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Stem Cells International
Volume 2016, Article ID 6810980, 14 pages
http://dx.doi.org/10.1155/2016/6810980
Research Article

Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method

1The Midwest Institute for Comparative Stem Cell Biotechnology, Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA
2Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, 35390 Giessen, Germany

Received 7 October 2015; Accepted 29 December 2015

Academic Editor: Shimon Slavin

Copyright © 2016 J. Robert Smith et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. L. Sensebé, M. Gadelorge, and S. Fleury-Cappellesso, “Production of mesenchymal stromal/stem cells according to good manufacturing practices: a review,” Stem Cell Research and Therapy, vol. 4, no. 3, article 66, 2013. View at Publisher · View at Google Scholar · View at Scopus
  2. M. Mendicino, A. M. Bailey, K. Wonnacott, R. K. Puri, and S. R. Bauer, “MSC-based product characterization for clinical trials: an FDA perspective,” Cell Stem Cell, vol. 14, no. 2, pp. 141–145, 2014. View at Publisher · View at Google Scholar · View at Scopus
  3. M. Krampera, J. Galipeau, Y. Shi, K. Tarte, and L. Sensebe, “Immunological characterization of multipotent mesenchymal stromal cells—the International Society for Cellular Therapy (ISCT) working proposal,” Cytotherapy, vol. 15, no. 9, pp. 1054–1061, 2013. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006. View at Publisher · View at Google Scholar · View at Scopus
  5. O. Ringdén, T. Erkers, S. Nava et al., “Fetal membrane cells for treatment of steroid-refractory acute graft-versus-host disease,” STEM CELLS, vol. 31, no. 3, pp. 592–601, 2013. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Duijvestein, A. C. W. Vos, H. Roelofs et al., “Autologous bone marrow-derived mesenchymal stromal cell treatment for refractory luminal Crohn's disease: results of a phase I study,” Gut, vol. 59, no. 12, pp. 1662–1669, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. J. P. McGuirk, J. R. Smith, C. L. Divine, M. Zuniga, and M. L. Weiss, “Wharton's Jelly-derived mesenchymal stromal cells as a promising cellular therapeutic strategy for the management of Graft-versus-host disease,” Pharmaceuticals, vol. 8, no. 2, pp. 196–220, 2015. View at Publisher · View at Google Scholar
  8. ClinicalTrials.gov, “Search of: mesenchymal stem cells,” ClinicalTrials.gov, 2015, https://clinicaltrials.gov/ct2/results?term=mesenchymal+stem+cells.
  9. A. Bersenev, “Cell therapy clinical trials—2014 report,” Cell Trials Blog, 2015, http://celltrials.info/2015/01/22/2014-report/.
  10. D. Baksh, R. Yao, and R. S. Tuan, “Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow,” STEM CELLS, vol. 25, no. 6, pp. 1384–1392, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. R. N. Barcia, J. M. Santos, M. Filipe et al., “What makes umbilical cord tissue-derived mesenchymal stromal cells superior immunomodulators when compared to bone marrow derived mesenchymal stromal cells?” Stem Cells International, vol. 2015, Article ID 583984, 14 pages, 2015. View at Publisher · View at Google Scholar
  12. L. Wang, I. Tran, K. Seshareddy, M. L. Weiss, and M. S. Detamore, “A comparison of human bone marrow-derived mesenchymal stem cells and human umbilical cord-derived mesenchymal stromal cells for cartilage tissue engineering,” Tissue Engineering—Part A, vol. 15, no. 8, pp. 2259–2266, 2009. View at Publisher · View at Google Scholar · View at Scopus
  13. K. H. Yoo, I. K. Jang, M. W. Lee et al., “Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues,” Cellular Immunology, vol. 259, no. 2, pp. 150–156, 2009. View at Publisher · View at Google Scholar · View at Scopus
  14. H. Zhang, S. Fazel, H. Tian et al., “Increasing donor age adversely impacts beneficial effects of bone marrow but not smooth muscle myocardial cell therapy,” American Journal of Physiology—Heart and Circulatory Physiology, vol. 289, no. 5, pp. H2089–H2096, 2005. View at Publisher · View at Google Scholar · View at Scopus
  15. Z.-Y. Zhang, S.-H. Teoh, M. S. K. Chong et al., “Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells,” STEM CELLS, vol. 27, no. 1, pp. 126–137, 2009. View at Publisher · View at Google Scholar · View at Scopus
  16. T. Deuse, M. Stubbendorff, K. Tang-Quan et al., “Immunogenicity and immunomodulatory properties of umbilical cord lining mesenchymal stem cells,” Cell Transplantation, vol. 20, no. 5, pp. 655–667, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. M. L. Weiss, C. Anderson, S. Medicetty et al., “Immune properties of human umbilical cord Wharton's jelly-derived cells,” Stem Cells, vol. 26, no. 11, pp. 2865–2874, 2008. View at Publisher · View at Google Scholar · View at Scopus
  18. M. Zeddou, A. Briquet, B. Relic et al., “The umbilical cord matrix is a better source of mesenchymal stem cells (MSC) than the umbilical cord blood,” Cell Biology International, vol. 34, no. 7, pp. 693–701, 2010. View at Publisher · View at Google Scholar · View at Scopus
  19. Y. Wang, Z.-B. Han, Y.-P. Song, and Z. C. Han, “Safety of mesenchymal stem cells for clinical application,” Stem Cells International, vol. 2012, Article ID 652034, 4 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  20. R. R. Sharma, K. Pollock, A. Hubel, and D. McKenna, “Mesenchymal stem or stromal cells: a review of clinical applications and manufacturing practices,” Transfusion, vol. 54, no. 5, pp. 1418–1437, 2014. View at Publisher · View at Google Scholar · View at Scopus
  21. A. Bongso and C.-Y. Fong, “The therapeutic potential, challenges and future clinical directions of stem cells from the Wharton's jelly of the human umbilical cord,” Stem Cell Reviews and Reports, vol. 9, no. 2, pp. 226–240, 2013. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Marmotti, S. Mattia, M. Bruzzone et al., “Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: an in vitro study,” Stem Cells International, vol. 2012, Article ID 326813, 13 pages, 2012. View at Publisher · View at Google Scholar · View at Scopus
  23. Y.-F. Han, R. Tao, T.-J. Sun, J.-K. Chai, G. Xu, and J. Liu, “Optimization of human umbilical cord mesenchymal stem cell isolation and culture methods,” Cytotechnology, vol. 65, no. 5, pp. 819–827, 2013. View at Publisher · View at Google Scholar · View at Scopus
  24. K. Seshareddy, D. Troyer, and M. L. Weiss, “Method to isolate mesenchymal-like cells from Wharton's Jelly of umbilical cord,” Methods in Cell Biology, vol. 86, pp. 101–119, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. Y. A. Romanov, V. A. Svintsitskaya, and V. N. Smirnov, “Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord,” STEM CELLS, vol. 21, no. 1, pp. 105–110, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. R. Sarugaser, D. Lickorish, D. Baksh, M. M. Hosseini, and J. E. Davies, “Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors,” STEM CELLS, vol. 23, no. 2, pp. 220–229, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. Y. López, K. Seshareddy, E. Trevino, J. Cox, and M. L. Weiss, “Evaluating the impact of oxygen concentration and plating density on human wharton's jelly-derived mesenchymal stromal cells,” Open Tissue Engineering and Regenerative Medicine Journal, vol. 4, no. 1, pp. 82–94, 2011. View at Publisher · View at Google Scholar · View at Scopus
  28. M. Reyes, A. Dudek, B. Jahagirdar, L. Koodie, P. H. Marker, and C. M. Verfaillie, “Origin of endothelial progenitors in human postnatal bone marrow,” Journal of Clinical Investigation, vol. 109, no. 3, pp. 337–346, 2002. View at Publisher · View at Google Scholar · View at Scopus
  29. S. Castiglia, K. Mareschi, L. Labanca et al., “Inactivated human platelet lysate with psoralen: a new perspective for mesenchymal stromal cell production in good manufacturing practice conditions,” Cytotherapy, vol. 16, no. 6, pp. 750–763, 2014. View at Publisher · View at Google Scholar · View at Scopus
  30. H. Hemeda, B. Giebel, and W. Wagner, “Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells,” Cytotherapy, vol. 16, no. 2, pp. 170–180, 2014. View at Publisher · View at Google Scholar · View at Scopus
  31. T. Burnouf, D. Strunk, M. B. Koh, and K. Schallmoser, “Human platelet lysate: replacing fetal bovine serum as a gold standard for human cell propagation?” Biomaterials, vol. 76, pp. 371–387, 2016. View at Publisher · View at Google Scholar
  32. K. Schallmoser, E. Rohde, C. Bartmann, A. C. Obenauf, A. Reinisch, and D. Strunk, “Platelet-derived growth factors for GMP-compliant propagation of mesenchymal stromal cells,” Bio-Medical Materials and Engineering, vol. 19, no. 4-5, pp. 271–276, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. R. Fazzina, P. Iudicone, A. Mariotti et al., “Culture of human cell lines by a pathogen-inactivated human platelet lysate,” Cytotechnology, 2015. View at Publisher · View at Google Scholar
  34. K. E. Mitchell, M. L. Weiss, B. M. Mitchell et al., “Matrix cells from Wharton's jelly form neurons and glia,” STEM CELLS, vol. 21, no. 1, pp. 50–60, 2003. View at Publisher · View at Google Scholar · View at Scopus
  35. D. L. Troyer and M. L. Weiss, “Concise review: Wharton's Jelly-derived cells are a primitive stromal cell population,” STEM CELLS, vol. 26, no. 3, pp. 591–599, 2008. View at Publisher · View at Google Scholar · View at Scopus
  36. P. Solves, R. Moraga, E. Saucedo et al., “Comparison between two strategies for umbilical cord blood collection,” Bone Marrow Transplantation, vol. 31, no. 4, pp. 269–273, 2003. View at Publisher · View at Google Scholar · View at Scopus
  37. E. J. Noh, Y. H. Kim, M. K. Cho, J. W. Kim, Y. J. Byun, and T.-B. Song, “Comparison of oxidative stress markers in umbilical cord blood after vaginal and cesarean delivery,” Obstetrics & Gynecology Science, vol. 57, no. 2, pp. 109–114, 2014. View at Publisher · View at Google Scholar
  38. M. François, I. B. Copland, S. Yuan, R. Romieu-Mourez, E. K. Waller, and J. Galipeau, “Cryopreserved mesenchymal stromal cells display impaired immunosuppressive properties as a result of heat-shock response and impaired interferon-γ licensing,” Cytotherapy, vol. 14, no. 2, pp. 147–152, 2012. View at Publisher · View at Google Scholar · View at Scopus
  39. D. J. Prockop, I. Sekiya, and D. C. Colter, “Isolation and characterization of rapidly self-renewing stem cells from cultures of human marrow stromal cells,” Cytotherapy, vol. 3, no. 5, pp. 393–396, 2001. View at Publisher · View at Google Scholar · View at Scopus