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Stem Cells International
Volume 2016, Article ID 6810980, 14 pages
Research Article

Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method

1The Midwest Institute for Comparative Stem Cell Biotechnology, Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA
2Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, 35390 Giessen, Germany

Received 7 October 2015; Accepted 29 December 2015

Academic Editor: Shimon Slavin

Copyright © 2016 J. Robert Smith et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplemental figures show the surface marker expression for six UC-MSC isolates and additional CFU-F information. Table 1 contains the surface marker expression data for six isolates. Note that all isolates conform to ISCT MSC surface marker criteria. In Table 2, the surface marker expression of UC-MSC isolates which have been frozen and never frozen was compared. Note that freezing did not significantly impact surface marker expression. Supplemental Figure 1 shows CFU-F expression of UC-MSCs evaluated at different times after plating. Based upon this experiment, day 6 at 5 cell per cm2 appears to yield the highest colony forming efficiency.

  1. Supplementary Material