Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method
Table 1
Data from 24 umbilical cord MSC isolations.
UC-MSC line
Sex
Birth
Length (cm)
Enzyme
Weight (g)
Gram per cm
Viability
±SE
Live cells per cm
±SE
Live cells per gram
±SE
Theoretical cell yield
241
F
V
46
High
67.9
1.5
76.7%
0.6%
242
M
V
43
High
60.7
1.4
58.0%
1.9%
243
F
V
57
High
81.1
1.4
62.8%
2.0%
244
M
C-S
35
High
66.0
1.9
58.6%
2.6%
245
M
V
61
High
76.0
1.2
50.6%
6.4%
246
M
C-S
41
High
60.9
1.5
66.0%
0.9%
248
F
C-S
47
High
72.6
1.5
68.3%
3.2%
249
F
C-S
32
High
37.7
1.2
64.2%
10.7%
250
M
V
26
High
43.3
1.7
84.2%
0.5%
251
F
V
28
High
30.4
1.1
74.4%
3.8%
252
M
C-S
54
High
83.6
1.5
79.8%
3.9%
253
M
V
61
Low
48.8
0.8
58.0%
3.7%
254
F
C-S
38
Low
59.3
1.6
60.9%
3.9%
255
F
C-S
47
Low
82.3
1.8
75.1%
8.6%
256
M
C-S
45
Low
49.0
1.1
70.2%
1.1%
257
M
C-S
43
Low
105.2
2.4
66.6%
3.0%
258
M
C-S
37
Low
45.1
1.2
62.5%
4.6%
259
M
C-S
31
Low
57.8
1.9
62.2%
3.5%
260
F
C-S
67
Low
100.6
1.5
64.4%
1.8%
261
M
C-S
51
Low
92.8
1.8
72.3%
2.1%
262
F
C-S
28.5
Low
25.1
0.9
50.5%
4.1%
263
F
C-S
28
Low
23.8
0.9
65.3%
3.8%
264
F
C-S
32
Low
45.7
1.4
59.8%
3.4%
265
M
C-S
38
Low
38.7
1.0
54.8%
3.2%
All 24 cords were compared below
Length
Weight
g per cm
Viability
Live cells per cm
Live cells per gram
Theo. yield
Mean
42.4
60.6
1.4
65.25%
Standard dev.
11.6
22.9
0.4
8.33%
Coefficiency of var.
27.5%
37.7%
26.9%
12.8%
47.9%
43.5%
49.7%
F = female, M = male, C-S = Caesarean-section, and V = vaginal. The enzyme concentration: low was 300 U/mL and high was 532 U/mL of collagenase. SE = standard error, which was calculated after averaging the technical replicates for each umbilical cord. Live cells per gram were calculated from the live cell number for each tube divided by the weight of the tube. Theoretical yield calculation represents cell numbers achieved assuming the entire umbilical cord was processed and expanded.
