Extracellular Matrix-Dependent Generation of Integration- and Xeno-Free iPS Cells Using a Modified mRNA Transfection Method

Figure 1

RNA-mediated generation of iPS cells using an ECM-based xeno-free/feeder-free hPSC medium. (a) A schematic representation of the experimental design. Human adult dermal fibroblasts were seeded on 6-well plates ( cells per well) and were subjected to multiple transfections as shown in the schedule. (b) The ESC-like colonies began to appear on day 11 after cell seeding (see the tip of the arrow at the center of the white circle, top right panel). The number within the square at the right bottom corner of each panel indicates the number of days passed after cell seeding. Scale bar: 100 µm. (c) The graph shows the number of ESC-like colonies generated per 10,000 fibroblasts initially seeded. (d) The percentage of ESC-like colonies among the total colonies is presented in graph format.

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