Extracellular Matrix-Dependent Generation of Integration- and Xeno-Free iPS Cells Using a Modified mRNA Transfection Method

Figure 3

qRT-PCR analyses of the iPS cells. (a) The mRNA-iPSCs were analyzed for the expression of multiple representative lineage-specific markers. H9-hESCs and urine-derived iPS cells generated using the episomal plasmid method (UNFiPSC1) [18] were used as positive controls, and EBs were used as negative controls. . (b–d) The expression levels of representative markers of derivatives of ectoderm (NCAM, Nestin, and Pax6) (b), mesoderm (FoxF1, Hand1, and Gata2) (c), and endoderm (AFP and GATA6) (d) were examined via qRT-PCR. In these experiments, H9-hESCs and UNFiPSC1 were used as controls for undifferentiated cells, whereas EBs and fibroblasts were used as controls for differentiated cells. .

(a)

(b)

(c)

(d)