Umbilical Cord as Prospective Source for Mesenchymal Stem Cell-Based Therapy

Figure 2

The characteristics of cultured cells derived from Wharton’s jelly according to minimal criteria to define human MSCs proposed by ISCT. (a) Analysis of immunophenotype with BD Stemflow™ hMSC Analysis Kit (BD Biosciences). Negative MSC cocktail includes PE CD45, PE CD34, PE CD11b, PE CD19, and PE HLA-DR antibody conjugates. (b) Phase contrast capture of UC-MSCs at the fourth passage. Scale bar: 200 μm. (c) Adipogenic differentiation with StemPro® Adipogenesis Differentiation Kit (Gibco). Lipid droplets are stained with Sudan III. Scale bar: 200 μm. (d) Osteogenic differentiation with StemPro Osteogenesis Differentiation Kit (Gibco). Calcificated nodules are stained with Alizarin red S (). Scale bar: 200 μm. (e) Chondrogenic differentiation with StemPro Chondrogenesis Differentiation Kit (Gibco). Mucopolysaccharides are stained with Alcian blue (). Scale bar: 200 μm.

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