Characterization of Neurogenic Potential of Dental Pulp Stem Cells Cultured in Xeno/Serum-Free Condition: In Vitro and In Vivo Assessment

Figure 6

Immunohistofluorescence findings of the neonatal brain (a) and injured spinal cord tissues (b) of rats at 1 week after transplantation of NSC medium cells. The tissues were stained with human nuclei (HN, green, (a)(F) and (b)(B); GFAP, red, (a)(G) and (b)(G); nestin, green, (a)(B) and (b)(F); and Sox2, red, (a)(C) and (b)(C)), and nuclear counterstaining was performed with DAPI (blue). (a)(B–D), (a)(F–H), (b)(B–D), and (b)(F–H) show magnified images of yellow-line boxes in (a)(A), (a)(E), (b)(A), and (b)(E) images, respectively. White scale bars = 100 μm; yellow scale bar = 20 μm.

(a)

(b)