Characterization of Neurogenic Potential of Dental Pulp Stem Cells Cultured in Xeno/Serum-Free Condition: In Vitro and In Vivo Assessment

Table 1

Sequence of primers used for quantitative real-time PCR in this study.

Gene	Sequence
Dcx	F: 5′-AGC CAA GAG CCC TGG TCC TAT-3′
	R: 5′-TGG AGG TTC CGT TTG CTG AGT-3′
Fibronectin	F: 5′-CAG TGG GAG ACC TCG AGA AG-3′
	R: 5′- CAA AGA CTA CAA GGC TCC CT-3′
Map2	F: 5′- AAC CCT TTG AGA ACA CGA CA-3′
	R: 5′-TCT TTC CGT TCA TCT GCC A-3′
Mash1	F: 5′-CCA GTT GTA CTT CAG CAC C-3′
	R: 5′-TGC CAC TTT GAG TTT GGA C-3′
NCAD	F: 5′-ACA GTG GCC ACC TAC AAA GG-3′
	R: 5′- GTA ATA GTT GGG GTA GAG CC-3′
NCAM	F: 5′-CAG CCA GCA GAT TAC AAT GC-3′
	R: 5′-TGG CTG GGA ACA ATA TCC AC-3′
Nestin	F: 5′-CTG GAG CAG GAG AAA CAG G-3′
	R: 5′-TGG GAG CAA AGA TCC AAG AC-3′
NeuroD1	F: 5′- CCA CGG ATC AAT CTT CTC AG-3′
	R: 5′-CAT GAT GTG AAT GGC TAT CG-3′
Olig2	F: 5′-GGT AAG TGC GCA ATG CTA AGC TGT-3′
	R: 5′-TAC AAA GCC CAG TTT GCA ACG CAG-3′
Pax6	F: 5′-ATG TGT GAG TAA AAT TCT GGG CA-3′
	R: 3′-GCT TAC AAC TTC TGG AGT CGC TA-3′
Sox1	F: 5′-AAT TTT ATT TTC GGC GTT GC-3′
	R: 5′-TGG GCT CTG TCT CTT AAA TTT GT-3′
Sox2	F: 5′-CCC AGC AGA CTT CAC ATG T-3′
	R: 5′-CCT CCC ATT TCC CTC GTT TT-3′
Vimentin	F: 5′-GAG AAC TTT GCC GTT GAA GC-3′
	R: 5′- CTA ACG GTG GAT GTC CTT CG -3′
18S rRNA	F: 5′-CGG CTA CAT CCA AGG AA-3′
	R: 5′-GCT GGA ATT ACC GCG GCT-3′

Dcx: doublecortin; Map2: microtubule associated protein 2; NeuroD1: neuronal differentiation 1; NCAD: neural cadherin; NCAM: neural cell adhesion molecule; Olig2: oligodendrocyte transcription factor 2; Pax6: paired box protein 6; Sox: sex-determining region Y-box.