PTF1a Activity in Enriched Posterior Foregut Endoderm, but Not Definitive Endoderm, Leads to Enhanced Pancreatic Differentiation in an In Vitro Mouse ESC-Based Model

Figure 2

Markers of DE are highly expressed in Nodal and Activin A treated cells, whereas markers of other lineages, including ectoderm, mesoderm, and extraembryonic endoderm are repressed. (a) DE markers, Sox17 and Foxa2, are upregulated in contrast to the genes of other germ layers, ectoderm (Sox1), mesoderm (Meox1), and extraembryonic endoderm (Sox7) in Nodal and Activin A treated cultures. . Data are presented as mean ± SEM. Asterisks indicate values on comparison with no-growth factor treated (No-GF) control cultures: , , and determined by one-way ANOVA with Tukey’s multiple comparison test. (b) Activin A and Nodal treatment lead to substantial number of FOXA2⁺SOX17⁺ cells, whereas the No-GF control cultures generate FOXA2⁺SOX17⁻ populations. Scale bars, 100 μm.

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