PTF1a Activity in Enriched Posterior Foregut Endoderm, but Not Definitive Endoderm, Leads to Enhanced Pancreatic Differentiation in an In Vitro Mouse ESC-Based Model

Figure 5

Pancreatic differentiation protocol adapted from Sneddon et al. [25]. Cells were seeded at a density of 60,000 cells/cm² either on Gelatin or on Matrigel. PTF1a was induced by adding 1 μg/mL Dox for 4 days overlapping with the end of primitive foregut endoderm stage (PF) and pancreatic endoderm stage (PE). Following the PE stage, cultures were further treated with Alk5 inhibitor and Nicotinamide (a maturation factor) for 8 days and analyzed for markers specific to adult pancreatic cell types.