PTF1a Activity in Enriched Posterior Foregut Endoderm, but Not Definitive Endoderm, Leads to Enhanced Pancreatic Differentiation in an In Vitro Mouse ESC-Based Model

Figure 8

High magnification images of Suspension-DE Dox cultures. (a, b) Horm⁺ and Amy⁺ cells are intermingled with each other. In particular amylase and hormone expression are cytoplasmic and do not overlap. (c) Insulin⁺ cells are mostly monohormonal but comingled with glucagon⁺ cells. (d) Insulin⁺ cells express PDX1 in the nucleus. Inset shows higher magnification to indicate nuclear PDX1 in insulin⁺ cells. Arrows point to many insulin⁺ cell clusters that have nuclear PDX1. Scale bars, 50 μm.

(a)

(b)

(c)

(d)