Heart-Derived Stem Cells in Miniature Swine with Coronary Microembolization: Novel Ischemic Cardiomyopathy Model to Assess the Efficacy of Cell-Based Therapy

Figure 5

The effect of CDCs on proliferating myocytes. (a) The upper panel shows a Ki67 positive nucleus localized in a cardiac myocyte. Two Ki67 positive nuclei (green, arrows) colocalized with myocytes stained with Troponin I (red). The number of cardiac myocytes in the growth phase of the cell cycle was evaluated with Ki67. Four weeks after CDCs, Ki67 positive myocytes were significantly increased in both LAD and RCA remote regions versus untreated controls. (b) pHH3 positive nuclei (green, arrow) colocalized with myocytes (red). Cardiac myocytes in the mitotic phase were evaluated with phospho-Histone H3 (pHH3). Four weeks after CDCs, myocyte nuclei in the mitotic phase significantly increased in both LAD and RCA remote regions versus untreated controls.

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