Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering

Figure 4

The expression of SMCs markers in human dental pulp stem cell (DPSC) clone A32 after SMC-induction. The A32 were induced in the SMCs induction protocol of 20% conditioned medium and 2.5 ng/mL TGF-β1 for the indicated time (0 d, 5 d, 8 d, 11 d, and 14 d). SMCs were regarded as a positive group. Noninduced A32 was regards as negative control. The mRNA expression of α-SMA, myosin, desmin, and calponin was analysed by qRT-PCR ((a)–(d)) and the protein levels of α-SMA, myosin, desmin were analysed by western blotting (e). The relative band intensities were determined by densitometry ((f)–(h)). Statistical analysis was performed by using one-way ANOVA. Date are shown as means ± SE. when compared with the 0 D group.

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