Research Article
Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering
| | Genes | Forward and reverse primers | Accession number |
| | GAPDH | 5′-GCACCGTCAAGGCTGAGAAC-3′ | NM_002046.3 | | 5′-TGGTGAAGACGCCAGTGGA-3′ |
| | CD105 | 5′-GAAACAGTCCATTGTGACCTTCAG-3′ | NM_001114753.2 | | 5′-GATGGCAGCTCTGTGGTGTTGACC-3′ |
| | CD73 | 5′-GTCGCGAACTTGCGCCTGGCCGCCAAG-3′ | NM_001204813.1 | | 5′-TGCAGCGGCTGGCGTTGACGCACTTGC-3′ |
| | CD44 | 5′-CATCTACCCCAGCAACCCTA-3′ | NM_000610.3 | | 5′-CTGTCTGTGCTGTCGGTGAT-3′ |
| | CD90 | 5′-ATGAACCTGGCCATCAGCATCG-3′ | NM_006288.3 | | 5′-CACGAGGTGTTCTGAGCCAGCA-3′ |
| | CD34 | 5′-ACAGGAGAAAGGCTGGGCGAAGACCCT-3′ | NM_001025109.1 | | 5′-TCCCCTGGGGGTTCCTGTATTGCGGCA-3′ |
| | α-SMA (ACTA2) | 5′-CCGGTTGGCCTTGGGGTTCAGGGGTGCC-3′ | NM_001141945.1 | | 5′-TCTCTCCAACCGGGGTCCCCCCTCCAGCG-3′ |
| | Myosin (MYH11) | 5′-AAGAAAGACACAAGTATCACGGGAGAGC-3′ | NM_001040113.1 | | 5′-TGTCACATTAATTCCCATGAGGTGGCAA-3′ |
| | Desmin | 5′-CACCATGAGCCAGGCCTACTCGTCCA-3′ | NM_001927.3 | | 5′-GGCAGCCAAATTGTTCTCTGCTTCTTCC-3′ |
| | Calponin | 5′-GGCTCCGTGAAGAAGATCAATGAGTCAA-3′ | NM_001299.4 | | 5′-CCCTAGGCGGAATTGTAGTAGTTGTGTG-3′ |
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