Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment

Figure 3

Expression of E-cadherin (a), MUC2 (b), vimentin (c), and -SMA (d) in human ALI tumor colorectal organoids. Comparison of a cancer stem cell marker, LGR5 expression in tumor organoids (e), and primary tumor tissues (f). The number of LGR5-positive cells in the pictures was counted ((g) n = 4, each for three patients (T1, T2, and T3)). versus organoid. Expression of CD44 (h) in human ALI tumor colorectal organoids. The paraffin sections (4 m) were immunofluorescently stained using antibody against E-cadherin, MUC2, vimentin, -SMA, LGR5, and CD44. Representative photomicrographs are shown (n = 4). Culture days 14–21. Scale bar: 50 m.

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