Real-Time Analysis of Endogenous Wnt Signalling in 3D Mesenchymal Stromal Cells

Figure 1

Stimulation using Wnt3a results in observable fluorescence in human Y201 MSC-EGFP reporter lines. (a) Overview of activated canonical Wnt signalling pathway. (b) Fluorescence microscopy of human Y201 MSC-EGFP reporter cells treated with varying concentrations of Wnt3a with or without a Wnt pathway inhibitor, IWR-1. Scale bar = 200 μm. (c) Flow cytometry scatter plot histograms of Wnt3a-treated Y201 Wnt EGFP reporters versus untreated, after removal of dead cells and debris by gating. Shift in population away from autofluorescence indicates positive EGFP expression (lower right quadrant).

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