Magnet-Bead Based MicroRNA Delivery System to Modify CD133⁺ Stem Cells

Figure 6

Viability and surface marker pattern after transfection. Cells were transfected with optimized miR/PEI (20 pmol miR; N/P ratio 7.5) and miR/PEI/MNP (20 pmol miR; N/P ratio 7.5; 3 and 5 μg/mL MNPs) complexes. Viability (a) and expression of CD45, CD34, and CD133 (b) were measured 18 h after transfection by flow cytometry. Untransfected cells (0 h and 18 h) were used as control. Values are presented as mean ± SEM; ; statistic was performed versus 0 h control (indicated as ) or versus 18 h control; ; ; .

(a)

(b)