Human Cord Blood-Derived CD133⁺/C-Kit⁺/Lin⁻ Cells Have Bipotential Ability to Differentiate into Mesenchymal Stem Cells and Outgrowth Endothelial Cells

Figure 1

Isolation and characterization of CKL− cells from human umbilical cord blood. (a) CKL− cells were isolated by density gradient centrifugation over Biocoll for 30 min at 400 ×g, washed three times in PBS, and purified by positive selection with anti-CD133/C-kit/Lin− microbeads using a magnetic cell sorter device. (b) Total mRNA was isolated from CKL− cells, and gene expression was assessed by RT-PCR. CKL− cells were further characterized by immunofluorescent staining for CD133 and C-Kit. (c, d) CKL− cells differentiated into OECs and MSCs as confirmed by RT-PCR and immunofluorescent staining for OEC- and MSC-specific markers.

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