Human Cord Blood-Derived CD133⁺/C-Kit⁺/Lin⁻ Cells Have Bipotential Ability to Differentiate into Mesenchymal Stem Cells and Outgrowth Endothelial Cells

Figure 3

Soluble factors generated from OECs or MSCs accelerate CKL− cell differentiation. (a) CKL− cells were cultured in OEC- or MSC-conditioned medium in a dose-dependent manner (0, 12.5, 25, or 50%). (b) The day of differentiation, defined as the first day on which differentiated colonies were observed from the time of seeding, occurred significantly sooner in CKL− cells differentiated in conditioned medium compared with control conditions. versus control. (c) GFP+ CKL− cells were cocultured with OECs or MSCs. Arrowheads indicate morphologically differentiated GFP⁺ OECs or MSCs. (d) OECs or MSCs differentiated from GFP⁺ CKL− cells were characterized by immunofluorescent staining using VE-cad or α-SMA antibody, respectively.

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