Human Cord Blood-Derived CD133⁺/C-Kit⁺/Lin⁻ Cells Have Bipotential Ability to Differentiate into Mesenchymal Stem Cells and Outgrowth Endothelial Cells

Figure 6

Functional characterization of OECs and MSCs. (a) Imaging of Dil-Ac-LDL-labeled OECs cultured on Matrigel-coated wells demonstrating capillary-like structure formation. (b) After incubation for 2-3 weeks in each differentiation medium, MSCs stained positively for calcium deposition (von Kossa staining), chondrocyte matrix (safranin-O staining), and lipid vacuoles (Oil Red O staining), indicating osteogenic, chondrogenic, and adipogenic differentiation, respectively. (c) Total mRNA was isolated from osteoblasts, chondrocytes, and adipocytes, and specific marker gene expression profiles were assessed by RT-PCR. OB: osteoblast, CD: chondrocyte, and AD: adipocyte.

(a)

(b)

(c)