Inhibition of Viability, Proliferation, Cytokines Secretion, Surface Antigen Expression, and Adipogenic and Osteogenic Differentiation of Adipose-Derived Stem Cells by Seven-Day Exposure to 0.5 T Static Magnetic Fields

Figure 5

Effect of seven-day exposure to 0.5 T SMF on cytokine secretion and stem cell genetic marker expression in ASCs and _SPIOASCs. (a) Representative results of RT-PCR for IGF-1, VEGF, and TGF-β1. (b) Quantification after normalization to levels from control ASCs. The expression of mRNA for IGF-1, VEGF, and TGF-β1 was significantly lower in the ASCs-SMF and _SPIOASCs-SMF than in ASCs and _SPIOASCs. (c) Representative results of RT-PCR for Sca1, ABCB1, and Oct-4. (d) Quantification after normalization to levels from control ASCs. The expression of mRNA for Sca1, ABCB1, and Oct-4 was significantly lower in the ASCs-SMF and _SPIOASCs-SMF than in ASCs and _SPIOASCs. versus ASCs or _SPIOASCs.

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