Inhibition of Viability, Proliferation, Cytokines Secretion, Surface Antigen Expression, and Adipogenic and Osteogenic Differentiation of Adipose-Derived Stem Cells by Seven-Day Exposure to 0.5 T Static Magnetic Fields

Figure 7

Effect of seven-day exposure to 0.5 T SMF on osteogenic differentiation in ASCs and _SPIOASCs. (a) Representative results of histochemical staining for alkaline phosphatase. (b) Quantification after normalization to levels from control ASCs. The alkaline phosphatase optical density value was significantly lower in the ASCs-SMF and _SPIOASCs-SMF than in ASCs and _SPIOASCs. (c) Representative results of RT-PCR for SPP and OSX. (d) Quantification after normalization to levels from control ASCs. The expression of mRNA for SPP and OSX was significantly lower in the ASCs-SMF and _SPIOASCs-SMF than in ASCs and _SPIOASCs. Scale bar represents 100 μm. versus ASCs or _SPIOASCs.

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