Stem Cells International / 2016 / Article / Tab 1 / Research Article
Inhibition of Viability, Proliferation, Cytokines Secretion, Surface Antigen Expression, and Adipogenic and Osteogenic Differentiation of Adipose-Derived Stem Cells by Seven-Day Exposure to 0.5 T Static Magnetic Fields Table 1 Primers of specific markers for transdifferentiation, cytokines, and stem cell genetic markers.
Gene Primer sequence Fragment, bp GenBank number LPL Forward 5′CACCTACACACAAGCAAAGCCCCA3′ 459 NM_012598 Reverse 5′GTGCTGTTCATCAGAGTGAGTTGGC3′ PPAR-γ Forward 5′CTACACCATGCTGGCCTCCCTGATG3′ 481 NM_013124 Reverse 5′TTGTCAGCGACTGGGACTTTTCTGC3′ SPP1 Forward 5′CGACAGTCAG GCGAGTTCCA AAGCC 3′ 470 NM_012881 Reverse 5′GCTGTTCCTG TAAGTTTGCC TGCCTC 3′ OSX Forward 5′AGTGGTGCAGGCAAACCTCCCCGGG 3′ 405 BK00141 Reverse 5′GACCTGGCTCCCCGTGGGTGCGCTG 3′ VEGF Forward 5′AGGTA CAGAG CAATG GGGCA GG 3′ 689 NM_03836 Reverse 5′ACGGA ATATC TCGGA AAACT GCTC 3′ IGF-1 Forward 5′CATGT CGTCT TCACA TCTCT TCTAC 3′ 299 NM_001082477 Reverse 5′CTTGT GTGTC GATAG GGGCT GGGAC 3′ TGF- Forward 5′AAGGCTCGCCAGTCCCCCGA3′ 400 NM_021578.2 Reverse 5′AGTGGGGGTCAGCAGCCGGT3′ OCT4 Forward 5′ ACCATCTGCCGCTTCGAGGC 3′ 526 NM_001009178 Reverse 5′ TGGCTCACCTCATCCCCAGG 3′ Sca1 Forward 5′CCCGTGGACCCTGCCAGTGC 3′ 761 X91619 Reverse 5′TGGCACCTGCGCACCCCTTC 3′ ABCB1 Forward 5′ GCCATGGCAGTGGGGCAGGT 3′ 600 NM_133401 Reverse 5′ GATGCGCTGCTTCTGCCCGC 3′ GAPDH Forward 5′ ATCTGACATGCCGCCTGGAGAAACC 3′ 287 NM_017008 Reverse 5′ CAGGGTTTCTTACTCCTTGGAGGCC 3′
LPL, Lipoprotein Lipase; PPAR-γ , Peroxisome Proliferator-Activated Receptor Gamma; SPP1, Secreted Phosphor-protein 1; OSX, Osterix; VEGF, Vascular Endothelial Growth Factor; IGF-1, Insulin-like Growth Factor-1; TGF-
