Improving Cell Engraftment in Cardiac Stem Cell Therapy

Figure 3

(a) Release kinetics of bFGF loaded in the hydrogels. bFGF loading was 10 and 50 μg/mL, respectively. The error bars are small. (b) VEGF expression of MSCs in the hydrogels under different culture conditions. Cells were cultured under conditions of 10% FBS and 21% oxygen, 1% FBS and 21% oxygen, 1% FBS and 5% oxygen, and 1% FBS and 1% oxygen, respectively. The expression was normalized by that under 10% FBS with 21% oxygen culture condition. (c) MSC survival in hydrogels cultured under different conditions. Culture conditions: 10% FBS and 21% oxygen, 1% FBS and 21% oxygen, 1% FBS and 5% oxygen, and 1% FBS and 1% oxygen. Double stranded DNA (dsDNA) content was used to quantify live cell number in the hydrogels. The dsDNA content at day 1 (100%) was used for normalization. This figure is adopted from [47].

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