Infusion of Bone Marrow Mesenchymal Stem Cells Attenuates Experimental Severe Acute Pancreatitis in Rats

Figure 2

(a) Typical cell shapes in primary culture, the adherent MSCs were triangular and fusiform and other cell shapes were also present (×200). P1 MSCs showed a fibroblast-like shape and whirlpool-like distribution (×100). Whirlpool-like distribution of the third passage of MSCs (×100). Embedded image shows P3 MSCs observed under phase contrast microscopy (×400). Flow cytometric analyses of CD29⁺CD44⁺CD45⁻ cells in MSCs at P0, P1, and P3; the percentage of Lin-CD29⁺CD44⁺CD45⁻ cells gated in R2 was 28.2%, 76.4%, and 96.4%, respectively. (b) Negative Oil Red O staining of control group MSCs at P3 MSCs were cultured in common medium before staining (×100). Positive Oil Red O staining of MSCs at P3 after 5 days of adipose differentiation induction; fat drops in MSCs are stained red (×100) in Negative Alizarin Red S staining of control group MSCs at P3; MSCs were cultured in common medium before staining (×100). Positive Alizarin Red S staining of MSCs at P3 after 14 days of osteocyte differentiation induction, calcium deposition in MSCs stained red (×100).

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