Allogeneic Platelet Releasate Preparations Derived via a Novel Rapid Thrombin Activation Process Promote Rapid Growth and Increased BMP-2 and BMP-4 Expression in Human Adipose-Derived Stem Cells

Figure 3

The effects of tPR on the growth of passage 1 ASCs. (a) 100,000 passage 0 ASCs were passaged and grown for 4 days in either 10% FBS or 10% tPR. Starting from an initial plating of 100,000 cells, tPR supplemented media displayed a significantly higher cell yield after four days (). (b) Little variability was seen in the population doubling times (PDT) in various ASC lines (A1–A5) exposed to different tPR preps (P1–P4). In particular, cell lines A4 and A5 were cultured in both P3 and tP4 PR preps and no significant differences in growth were seen.

(a)

(b)