Highly Efficient In Vitro Reparative Behaviour of Dental Pulp Stem Cells Cultured with Standardised Platelet Lysate Supplementation

Figure 3

In vitro migration capacity of DPSCs is enhanced by PL. (a) Representative images of early time points during the scratch assay. Images were taken 2 hrs and 24 hrs after the initial scratch and are representative of 3 independent experiments. The cells were fixed and stained at different endpoints as shown in (b) for 3 days and (c) 7 days. (d) Quantitative analysis of wound healing at 24 hrs after scratch. 100% is set as open area 2 h time points. Data shown as mean + SD, . Statistical significance determined using unpaired -test (). (e) Quantification of chemotaxis derived from under-agarose assay. Data were collected using 24 h time points light microscope images. Data shown as mean + SD, . Statistical significance determined using unpaired -test ().

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