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Stem Cells International
Volume 2016, Article ID 7231567, 13 pages
Research Article

Enhanced Ex Vivo Expansion of Human Hematopoietic Progenitors on Native and Spin Coated Acellular Matrices Prepared from Bone Marrow Stromal Cells

1CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India
2Deakin University, Waurn Ponds, Geelong, VIC 3220, Australia
3Sridevi Maternity and Nursing Home, Warasiguda, Hyderabad 500 361, India

Received 25 September 2015; Revised 13 December 2015; Accepted 5 January 2016

Academic Editor: Pedro M. Baptista

Copyright © 2016 Samiksha Wasnik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The extracellular microenvironment in bone marrow (BM) is known to regulate the growth and differentiation of hematopoietic stem and progenitor cells (HSPC). We have developed cell-free matrices from a BM stromal cell line (HS-5), which can be used as substrates either in native form or as tissue engineered coatings, for the enhanced ex vivo expansion of umbilical cord blood (UCB) derived HSPC. The physicochemical properties (surface roughness, thickness, and uniformity) of native and spin coated acellular matrices (ACM) were studied using scanning and atomic force microscopy (SEM and AFM). Lineage-specific expansion of HSPC, grown on these substrates, was evaluated by immunophenotypic (flow cytometry) and functional (colony forming) assays. Our results show that the most efficient expansion of lineage-specific HSPC occurred on spin coated ACM. Our method provides an improved protocol for ex vivo HSPC expansion and it offers a system to study the in vivo roles of specific molecules in the hematopoietic niche that influence HSPC expansion.