Enhanced Ex Vivo Expansion of Human Hematopoietic Progenitors on Native and Spin Coated Acellular Matrices Prepared from Bone Marrow Stromal Cells

Figure 1

Preparation of spin coated HS-5 acellular matrices. (a) Decellularization of HS-5 cells. (b) Physical characterization of the acellular matrices. (c) Spin coating process: step 1—extraction and collection of acellular matrices, step 2—sample kept on tissue culture surfaces to be coated, which is held in vacuum on a stub, step 3—spinning at fixed rpm, and step 4—coating and drying under sterile conditions.