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Stem Cells International
Volume 2016, Article ID 7231567, 13 pages
Research Article

Enhanced Ex Vivo Expansion of Human Hematopoietic Progenitors on Native and Spin Coated Acellular Matrices Prepared from Bone Marrow Stromal Cells

1CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India
2Deakin University, Waurn Ponds, Geelong, VIC 3220, Australia
3Sridevi Maternity and Nursing Home, Warasiguda, Hyderabad 500 361, India

Received 25 September 2015; Revised 13 December 2015; Accepted 5 January 2016

Academic Editor: Pedro M. Baptista

Copyright © 2016 Samiksha Wasnik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The efficiency of decellularization of the HS-5 stromal cells with 0.02 M NH4OH treatment was analyzed in phase microscope; matrices were stained to confirm the complete removal of nuclear material using DAPI, also, DNA content in HS-5 stromal cells and decellularized ACM was quantified (Figure S1). Table S1: Total matrix protein estimation after decellularization. The blank glass coverslips with no cells, native or spin coated matrix was taken as negative control for the surface topographical analysis in AFM (Figure S2). Table S2: The average thickness of native and spin coated matrices measured in AFM. The native and spin coated matrices were also characterized by cytochemical and immunofluorescence stainings for matrix proteins (Figures S3 and S4: Representative images of negative controls).

  1. Supplementary Material